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BIOTINYLATION

  • Biotinylation
  • Biotechnological modification process to attach Biotin to a molecule

    biochemistry, biotinylation is the process of covalently attaching biotin to a protein, nucleic acid or other molecule. Biotinylation is rapid, specific

    Biotinylation

    Biotinylation

  • Hi-C (genomic analysis technique)
  • Genomic analysis technique

    Hi-C is a high-throughput genomic and epigenomic technique to capture chromatin conformation (3C). In general, Hi-C is considered as a derivative of a

    Hi-C (genomic analysis technique)

    Hi-C (genomic analysis technique)

    Hi-C_(genomic_analysis_technique)

  • Biotin
  • Chemical compound and essential nutrient

    acids and fatty acids, synthesis of fatty acids, and gluconeogenesis. Biotinylation of histone proteins in nuclear chromatin plays a role in chromatin stability

    Biotin

    Biotin

    Biotin

  • Biotin—(acetyl-CoA-carboxylase) ligase
  • Enzyme catalyzing biotin metabolism

    that is optimal for dimerization and high-affinity DNA binding. The biotinylation reaction occurs in two discrete steps within the same active site: BirA

    Biotin—(acetyl-CoA-carboxylase) ligase

    Biotin—(acetyl-CoA-carboxylase)_ligase

  • Photobiotin
  • Chemical compound

    nonspecific labeling of proteins, DNA and RNA probes or other molecules. Biotinylation of DNA and RNA with photoactivatable biotin is easier and less expensive

    Photobiotin

    Photobiotin

    Photobiotin

  • Post-translational modification
  • Chemical changes in proteins following their translation from mRNA

    addition of a succinyl group to lysine biotinylation: covalent attachment of a biotin moiety using a biotinylation reagent, typically for the purpose of

    Post-translational modification

    Post-translational modification

    Post-translational_modification

  • Enzyme
  • Large biological molecule that acts as a catalyst

    ISBN 978-1-118-91840-1. Chapman-Smith A, Cronan JE (September 1999). "The enzymatic biotinylation of proteins: a post-translational modification of exceptional specificity"

    Enzyme

    Enzyme

    Enzyme

  • Luciferase
  • Family of proteins which produce bioluminescence

    assays. Luciferase can act as an ATP sensor protein through biotinylation. Biotinylation will immobilize luciferase on the cell-surface by binding to

    Luciferase

    Luciferase

    Luciferase

  • Proximity labeling
  • Laboratory technique

    N-terminus primary amines on neighboring proteins. Constitutive TurboID biotinylation with endogenous biotin and ATP may be cytotoxic in some cases. To label

    Proximity labeling

    Proximity labeling

    Proximity_labeling

  • Intrinsically disordered proteins
  • Protein without a fixed 3D structure

    2018 that biotinylation favors intrinsically disordered regions as they are more accessible. Although the association between biotinylation and disorder

    Intrinsically disordered proteins

    Intrinsically disordered proteins

    Intrinsically_disordered_proteins

  • Holoprotein
  • Enzyme linked with cofactor

    30 October 2007. Chapman-Smith A, Cronan JE (1999). "The enzymatic biotinylation of proteins: a post-translational modification of exceptional specificity"

    Holoprotein

    Holoprotein

  • ELISpot
  • Medical laboratory test

    that the antibody can bind to the new streptavidin-enzyme conjugate. Biotinylation basically creates a strong affinity between the biotin on the cytokine-specific

    ELISpot

    ELISpot

    ELISpot

  • Protein tag
  • Artificial peptide attached to protein for marking purpose

    not). Protein tags may allow specific enzymatic modification (such as biotinylation by biotin ligase) or chemical modification (such as coupling to other

    Protein tag

    Protein_tag

  • Protein folding
  • Change of a linear protein chain to a 3D structure

    PMC 3497811. PMID 22949695. Minde DP, Ramakrishna M, Lilley KS (2018). "Biotinylation by proximity labelling favours unfolded proteins". bioRxiv 10.1101/274761

    Protein folding

    Protein folding

    Protein_folding

  • Immunostaining
  • Biochemical technique

    rigorous optimisation of tissue fixation and processing methods. Protein biotinylation in vivo was proposed to alleviate the problems caused by frequent incompatibility

    Immunostaining

    Immunostaining

    Immunostaining

  • Chromatin immunoprecipitation
  • Genomic technique

    K. An epitope-tagged version of the protein of interest, or in vivo biotinylation can be used instead of antibodies to the native protein of interest

    Chromatin immunoprecipitation

    Chromatin immunoprecipitation

    Chromatin_immunoprecipitation

  • Citrullination
  • Biological process

    Citrulline residues can be chemically modified with butanedione or by biotinylation prior to analysis, leading to a different mass shift, and this strategy

    Citrullination

    Citrullination

    Citrullination

  • Chemical biology
  • Scientific discipline

    (August 2015). "A Cell-Permeable ATP Analogue for Kinase-Catalyzed Biotinylation". Angewandte Chemie. 54 (33): 9618–9621. doi:10.1002/anie.201503041

    Chemical biology

    Chemical biology

    Chemical_biology

  • TAAR1
  • Protein found in humans

    orientation of hTAAR1 proteins in cells. An acceptor peptide (AP) tag for biotinylation was added to hTAARI for quantification of cell surface hTAARI. The function

    TAAR1

    TAAR1

    TAAR1

  • Avidin
  • Type of protein

    be reversed through an intravenous infusion of avidin. Streptavidin Biotinylation Helppolainen SH, Nurminen KP, Määttä JA, Halling KK, Slotte JP, Huhtala

    Avidin

    Avidin

    Avidin

  • Adhesome
  • readily isolated from cells attached to glass. The advent of proximity biotinylation by birA* has facilitated the first proteomics-based studies of the cadherin

    Adhesome

    Adhesome

  • List of MeSH codes (G06)
  • The following is a partial list of the "G" codes for Medical Subject Headings (MeSH), as defined by the United States National Library of Medicine (NLM)

    List of MeSH codes (G06)

    List_of_MeSH_codes_(G06)

  • Dideoxynucleotide
  • Chain-elongating inhibitor of DNA polymerase

    used will likely occur by treatment with a phosphorylation enzyme and biotinylation and reaction of the biotinylated substance with the dye. It is possible

    Dideoxynucleotide

    Dideoxynucleotide

    Dideoxynucleotide

  • Eastern blot
  • Biochemical technique

    (myristoylation, palmitoylation), alkylation, arginylation, ADP-ribosylation, biotinylation, formylation, geranylgeranylation, glutamylation, glycosylation, glycylation

    Eastern blot

    Eastern_blot

  • Alice Y. Ting
  • American chemist

    Ting and her lab developed monovalent streptavidin, site-specific biotinylation in mammalian cells, small monovalent quantum dots for single molecule

    Alice Y. Ting

    Alice_Y._Ting

  • Biotin carboxyl carrier protein
  • antiparallel β-strands that wrap around a central hydrophobic core. The biotinylation motif Met-Lys-Met is located at the tip of the β-hairpin structure.

    Biotin carboxyl carrier protein

    Biotin carboxyl carrier protein

    Biotin_carboxyl_carrier_protein

  • Epitranscriptomic sequencing
  • resolution. In an optimisation step, azido-CMC can confer the ability to add biotinylation; subsequent biotin pulldown will enrich Ψ-containing transcripts, allowing

    Epitranscriptomic sequencing

    Epitranscriptomic sequencing

    Epitranscriptomic_sequencing

  • Holocarboxylase synthetase
  • Class of enzymes

    characteristic signs and symptoms of holocarboxylase synthetase deficiency. Biotinylation Siegel L, Foote JL, Coon MJ (March 1965). "The enzymatic synthesis of

    Holocarboxylase synthetase

    Holocarboxylase_synthetase

  • Olfactomedin 4
  • Protein-coding gene in the species Homo sapiens

    accessible tumor antigens in human colon cancer by ex vivo protein biotinylation and comparative mass spectrometry analysis". Int. J. Cancer. 123 (12):

    Olfactomedin 4

    Olfactomedin 4

    Olfactomedin_4

  • Nanopore sequencing
  • DNA / RNA sequencing technique

    has been to attach the exonuclease to the nanopore, perhaps through biotinylation to the beta barrel hemolysin. The central pore of the protein may be

    Nanopore sequencing

    Nanopore sequencing

    Nanopore_sequencing

  • Chem-seq
  • Technique for mapping interactions between molecules and their protein targets

    to be feasible, the small molecule under study must be amenable to biotinylation without disruption of its natural binding properties. This is simply

    Chem-seq

    Chem-seq

  • Bissulfosuccinimidyl suberate
  • Chemical compound

    B. T.; Collins, T. J.; Rogers, M. E.; Kurosky, A. (1997). "Peptide biotinylation with amine-reactive esters: differential side chain reactivity". Peptides

    Bissulfosuccinimidyl suberate

    Bissulfosuccinimidyl_suberate

  • Circulating tumor DNA
  • Tumor-derived fragmented DNA in the bloodstream

    Biotinylated primers designed to bind to the tags are used to amplify the DNA. Biotinylation allows the amplified DNA to bind to the magnetic beads, which are coated

    Circulating tumor DNA

    Circulating tumor DNA

    Circulating_tumor_DNA

  • Terminal amine isotopic labeling of substrates
  • proteolysis products. Acetylation of amines followed by tryptic digestion and biotinylation of free N-terminal peptides uses chemical (acetylation) to label free

    Terminal amine isotopic labeling of substrates

    Terminal_amine_isotopic_labeling_of_substrates

  • Magnetoelastic filament
  • coating, polyacrylic acid (PAA) coating, tetraethoxysilane condensation, biotinylation or glucose decomposition. Typically, the primary building blocks of

    Magnetoelastic filament

    Magnetoelastic_filament

  • Bioconjugation
  • Chemical process

    and unwanted reactivity. Immunofluorescence Biomolecular engineering Biotinylation SpyTag/SpyCatcher In situ cyclization of proteins Unnatural amino acids

    Bioconjugation

    Bioconjugation

    Bioconjugation

  • Beta-propeller protein-associated neurodegeneration
  • Neurodegenerative disorder with brain iron accumulation

    reactivation) in BPAN female patients' fibroblasts through histone biotinylation, a process where biotin is attached to histones. Researchers successfully

    Beta-propeller protein-associated neurodegeneration

    Beta-propeller_protein-associated_neurodegeneration

  • Off-target genome editing
  • Type of unintended effects of genetic modification techniques

    2013, BLESS is performed by ligating the DSB ends with biotin i.e. biotinylation. This is followed by separation/collection of said ligated ends using

    Off-target genome editing

    Off-target_genome_editing

  • Cofactor transferase family
  • Protein domain

    PMID 11106165. Chapman-Smith A, Cronan JE (September 1999). "The enzymatic biotinylation of proteins: a post-translational modification of exceptional specificity"

    Cofactor transferase family

    Cofactor transferase family

    Cofactor_transferase_family

  • Mir-153 microRNA precursor family
  • RNA family

    holocarboxylase synthetase, thereby increasing the abundance of histone H4 biotinylation marks in HEK-293 human kidney cells". The Journal of Nutritional Biochemistry

    Mir-153 microRNA precursor family

    Mir-153_microRNA_precursor_family

  • List of MeSH codes (E05)
  • site-directed MeSH E05.393.420.890 – sex preselection MeSH E05.393.525.085 – biotinylation MeSH E05.393.525.100 – blotting, northern MeSH E05.393.525.150 – blotting

    List of MeSH codes (E05)

    List_of_MeSH_codes_(E05)

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