Search references for BIOTINYLATION. Phrases containing BIOTINYLATION
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Biotechnological modification process to attach Biotin to a molecule
biochemistry, biotinylation is the process of covalently attaching biotin to a protein, nucleic acid or other molecule. Biotinylation is rapid, specific
Biotinylation
Genomic analysis technique
Hi-C is a high-throughput genomic and epigenomic technique to capture chromatin conformation (3C). In general, Hi-C is considered as a derivative of a
Hi-C (genomic analysis technique)
Hi-C_(genomic_analysis_technique)
Chemical compound and essential nutrient
acids and fatty acids, synthesis of fatty acids, and gluconeogenesis. Biotinylation of histone proteins in nuclear chromatin plays a role in chromatin stability
Biotin
Enzyme catalyzing biotin metabolism
that is optimal for dimerization and high-affinity DNA binding. The biotinylation reaction occurs in two discrete steps within the same active site: BirA
Biotin—(acetyl-CoA-carboxylase) ligase
Biotin—(acetyl-CoA-carboxylase)_ligase
Chemical compound
nonspecific labeling of proteins, DNA and RNA probes or other molecules. Biotinylation of DNA and RNA with photoactivatable biotin is easier and less expensive
Photobiotin
Chemical changes in proteins following their translation from mRNA
addition of a succinyl group to lysine biotinylation: covalent attachment of a biotin moiety using a biotinylation reagent, typically for the purpose of
Post-translational modification
Post-translational_modification
Large biological molecule that acts as a catalyst
ISBN 978-1-118-91840-1. Chapman-Smith A, Cronan JE (September 1999). "The enzymatic biotinylation of proteins: a post-translational modification of exceptional specificity"
Enzyme
Family of proteins which produce bioluminescence
assays. Luciferase can act as an ATP sensor protein through biotinylation. Biotinylation will immobilize luciferase on the cell-surface by binding to
Luciferase
Laboratory technique
N-terminus primary amines on neighboring proteins. Constitutive TurboID biotinylation with endogenous biotin and ATP may be cytotoxic in some cases. To label
Proximity_labeling
Protein without a fixed 3D structure
2018 that biotinylation favors intrinsically disordered regions as they are more accessible. Although the association between biotinylation and disorder
Intrinsically disordered proteins
Intrinsically_disordered_proteins
Enzyme linked with cofactor
30 October 2007. Chapman-Smith A, Cronan JE (1999). "The enzymatic biotinylation of proteins: a post-translational modification of exceptional specificity"
Holoprotein
Medical laboratory test
that the antibody can bind to the new streptavidin-enzyme conjugate. Biotinylation basically creates a strong affinity between the biotin on the cytokine-specific
ELISpot
Artificial peptide attached to protein for marking purpose
not). Protein tags may allow specific enzymatic modification (such as biotinylation by biotin ligase) or chemical modification (such as coupling to other
Protein_tag
Change of a linear protein chain to a 3D structure
PMC 3497811. PMID 22949695. Minde DP, Ramakrishna M, Lilley KS (2018). "Biotinylation by proximity labelling favours unfolded proteins". bioRxiv 10.1101/274761
Protein_folding
Biochemical technique
rigorous optimisation of tissue fixation and processing methods. Protein biotinylation in vivo was proposed to alleviate the problems caused by frequent incompatibility
Immunostaining
Genomic technique
K. An epitope-tagged version of the protein of interest, or in vivo biotinylation can be used instead of antibodies to the native protein of interest
Chromatin_immunoprecipitation
Biological process
Citrulline residues can be chemically modified with butanedione or by biotinylation prior to analysis, leading to a different mass shift, and this strategy
Citrullination
Scientific discipline
(August 2015). "A Cell-Permeable ATP Analogue for Kinase-Catalyzed Biotinylation". Angewandte Chemie. 54 (33): 9618–9621. doi:10.1002/anie.201503041
Chemical_biology
Protein found in humans
orientation of hTAAR1 proteins in cells. An acceptor peptide (AP) tag for biotinylation was added to hTAARI for quantification of cell surface hTAARI. The function
TAAR1
Type of protein
be reversed through an intravenous infusion of avidin. Streptavidin Biotinylation Helppolainen SH, Nurminen KP, Määttä JA, Halling KK, Slotte JP, Huhtala
Avidin
readily isolated from cells attached to glass. The advent of proximity biotinylation by birA* has facilitated the first proteomics-based studies of the cadherin
Adhesome
The following is a partial list of the "G" codes for Medical Subject Headings (MeSH), as defined by the United States National Library of Medicine (NLM)
List_of_MeSH_codes_(G06)
Chain-elongating inhibitor of DNA polymerase
used will likely occur by treatment with a phosphorylation enzyme and biotinylation and reaction of the biotinylated substance with the dye. It is possible
Dideoxynucleotide
Biochemical technique
(myristoylation, palmitoylation), alkylation, arginylation, ADP-ribosylation, biotinylation, formylation, geranylgeranylation, glutamylation, glycosylation, glycylation
Eastern_blot
American chemist
Ting and her lab developed monovalent streptavidin, site-specific biotinylation in mammalian cells, small monovalent quantum dots for single molecule
Alice_Y._Ting
antiparallel β-strands that wrap around a central hydrophobic core. The biotinylation motif Met-Lys-Met is located at the tip of the β-hairpin structure.
Biotin carboxyl carrier protein
Biotin_carboxyl_carrier_protein
resolution. In an optimisation step, azido-CMC can confer the ability to add biotinylation; subsequent biotin pulldown will enrich Ψ-containing transcripts, allowing
Epitranscriptomic_sequencing
Class of enzymes
characteristic signs and symptoms of holocarboxylase synthetase deficiency. Biotinylation Siegel L, Foote JL, Coon MJ (March 1965). "The enzymatic synthesis of
Holocarboxylase_synthetase
Protein-coding gene in the species Homo sapiens
accessible tumor antigens in human colon cancer by ex vivo protein biotinylation and comparative mass spectrometry analysis". Int. J. Cancer. 123 (12):
Olfactomedin_4
DNA / RNA sequencing technique
has been to attach the exonuclease to the nanopore, perhaps through biotinylation to the beta barrel hemolysin. The central pore of the protein may be
Nanopore_sequencing
Technique for mapping interactions between molecules and their protein targets
to be feasible, the small molecule under study must be amenable to biotinylation without disruption of its natural binding properties. This is simply
Chem-seq
Chemical compound
B. T.; Collins, T. J.; Rogers, M. E.; Kurosky, A. (1997). "Peptide biotinylation with amine-reactive esters: differential side chain reactivity". Peptides
Bissulfosuccinimidyl_suberate
Tumor-derived fragmented DNA in the bloodstream
Biotinylated primers designed to bind to the tags are used to amplify the DNA. Biotinylation allows the amplified DNA to bind to the magnetic beads, which are coated
Circulating_tumor_DNA
proteolysis products. Acetylation of amines followed by tryptic digestion and biotinylation of free N-terminal peptides uses chemical (acetylation) to label free
Terminal amine isotopic labeling of substrates
Terminal_amine_isotopic_labeling_of_substrates
coating, polyacrylic acid (PAA) coating, tetraethoxysilane condensation, biotinylation or glucose decomposition. Typically, the primary building blocks of
Magnetoelastic_filament
Chemical process
and unwanted reactivity. Immunofluorescence Biomolecular engineering Biotinylation SpyTag/SpyCatcher In situ cyclization of proteins Unnatural amino acids
Bioconjugation
Neurodegenerative disorder with brain iron accumulation
reactivation) in BPAN female patients' fibroblasts through histone biotinylation, a process where biotin is attached to histones. Researchers successfully
Beta-propeller protein-associated neurodegeneration
Beta-propeller_protein-associated_neurodegeneration
Type of unintended effects of genetic modification techniques
2013, BLESS is performed by ligating the DSB ends with biotin i.e. biotinylation. This is followed by separation/collection of said ligated ends using
Off-target_genome_editing
Protein domain
PMID 11106165. Chapman-Smith A, Cronan JE (September 1999). "The enzymatic biotinylation of proteins: a post-translational modification of exceptional specificity"
Cofactor_transferase_family
RNA family
holocarboxylase synthetase, thereby increasing the abundance of histone H4 biotinylation marks in HEK-293 human kidney cells". The Journal of Nutritional Biochemistry
Mir-153 microRNA precursor family
Mir-153_microRNA_precursor_family
site-directed MeSH E05.393.420.890 – sex preselection MeSH E05.393.525.085 – biotinylation MeSH E05.393.525.100 – blotting, northern MeSH E05.393.525.150 – blotting
List_of_MeSH_codes_(E05)
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