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DNA / RNA sequencing technique
Nanopore sequencing is a third generation approach used in the sequencing of biopolymers — specifically, polynucleotides in the form of DNA or RNA. Nanopore
Nanopore_sequencing
Pore of nanometer size
nanopore sequencing. Nanopore sequencing can occur with bacterial nanopores as mentioned in the above section as well as with the Nanopore sequencing
Nanopore
U.K.-based nanopore sequencing company
Oxford Nanopore Technologies plc is a UK-based company which develops and sells nanopore sequencing products (including the portable DNA sequencer, MinION)
Oxford_Nanopore_Technologies
DNA sequencing
generation sequencing, also known as next-generation sequencing methods. These methods emerged in 2008, characterized by technologies such as nanopore sequencing
Third-generation_sequencing
Sequencing all the DNA of an individual at once
[citation needed] Long-read sequencing techniques have emerged, including nanopore technology. Though the sequencing accuracy of Nanopore technology is lower
Whole_genome_sequencing
American cell biologist
genome. Despite earlier skepticism, nanopore sequencing is now accepted as a viable third generation sequencing method. Another major area of Deamer's
David_W._Deamer
Process of determining the nucleic acid sequence
development are solid state nanopore sequencing, and protein based nanopore sequencing. Protein nanopore sequencing utilizes membrane protein complexes
DNA_sequencing
Method of analysis of genetic material
previously undescribed or divergent pathogens. Sequencing Clinical data management Nanopore sequencing Govender, Kumeren N.; Street, Teresa L.; Sanderson
Clinical metagenomic sequencing
Clinical_metagenomic_sequencing
Scientific instrument that automates the DNA sequencing process
third-generation DNA sequencers such as PacBio SMRT and Oxford Nanopore offer the possibility of sequencing long molecules, compared to short-read technologies such
DNA_sequencer
Base callers for Nanopore sequencing like Guppy or Dorado, use neural networks trained on current signals obtained from accurate sequencing data. Base calling
Base_calling
are deaminated, thereby being read-out as thymines during sequencing. Nanopore sequencing has emerged as an alternative platform for fragmentome analysis
Fragmentomics
Professor of Microbiology
smegmatis porin A (MspA) was the basis for a DNA sequencing technology known as nanopore sequencing. Niederweis was born and raised in Germany. He graduated
Michael_Niederweis
Novel genomic technique
utilizes chromatin conformation capture (3C) and Oxford Nanopore Technologies' (ONT) long-read sequencing to characterize three-dimensional (3D) chromatin structure
Pore-C
Examines sequence information from individual cells
Nanopore-based sequencing also offers a route for direct methylation sequencing without fragmentation or modification to the original DNA. Nanopore sequencing
Single-cell_sequencing
American geneticist (born 1954)
1994. Church was co-inventor of nanopore sequencing in 1995, which is now commercially available (e.g. Oxford Nanopore Technologies), but not in the form
George_Church_(geneticist)
Lab technique in cellular biology
technologies for cDNA sequencing including platforms developed by Illumina, Thermo Fisher, BGI/MGI, PacBio, and Oxford Nanopore Technologies. For Illumina
RNA-Seq
Spanish physicist
minION device developed by Oxford Nanopore Technologies. The researchers only needed two picograms for the sequencing. This advance suggests that super-tiny
Javier_Martín-Torres
technologies such as Oxford Nanopore sequencing. Libraries produced by linked-read can be processed using Illumina short read sequencing, increasing accessibility
Linked-read_sequencing
Previously known single-molecule sequencing technologies include Nanopore sequencing (Oxford Nanopore), SMRT sequencing (Pacific Biosciences), and Heliscope
Single-molecule magnetic sequencing
Single-molecule_magnetic_sequencing
DNA sequencing method
status during bioinformatics analysis. More recently, nanopore or single-molecule realtime sequencing have enabled direct detection of methylated cytosines
5-base_sequencing
Method of DNA sequencing developed in 1977
Sanger sequencing is a method of DNA sequencing that involves electrophoresis and is based on the random incorporation of chain-terminating dideoxynucleotides
Sanger_sequencing
modifications by SMRT sequencing is the direct detection using the Nanopore sequencing technologies. This technique exploits nanometer-sized protein channels
Epitranscriptomic_sequencing
Study of RNA transcripts
RNA-Seq. Direct sequencing of RNA using nanopore sequencing represents a current state-of-the-art RNA-Seq technique. Nanopore sequencing of RNA can detect
Transcriptomics_technologies
Intergenic DNA sequence separating ribosomal RNA genes
basidiomycete ITS sequences from mycorrhiza samples). Despite shotgun sequencing and nanopore sequencing methods becoming increasingly utilized in medical microbiology
Internal_transcribed_spacer
British entrepreneur and programmer
community COVID-19 infection rates in the UK using serology, PCR and Nanopore Sequencing Technology". Oxford Department of Zoology. 4 April 2020. Archived
Ben_Delo
and infrastructure. For example, Nanopore sequencing Technology currently provides the ability to do rapid DNA sequencing with a handheld device. Hence,
Medical_genetic_ethics
lipid bilayer. This was an important milestone in the development of nanopore sequencing. Bhakdi S, Tranum-Jensen J (December 1991). "Alpha-toxin of Staphylococcus
Staphylococcus aureus alpha toxin
Staphylococcus_aureus_alpha_toxin
Genome Consortium Ion semiconductor sequencing Nanopore sequencing Next-generation sequencing Oncogenomics Polony sequencing Precision medicine Pyrosequencing
Cancer_genome_sequencing
American biotechnology company
promising genetic-sequencing technology". Nature nature.2016.19451. doi:10.1038/nature.2016.19451. S2CID 211836642. "Illumina sues Oxford Nanopore for patent
Illumina,_Inc.
Method of DNA sequence reconstruction
Biosystems, Ion Torrent and SMRT were released and new technologies (e.g. Nanopore sequencing) continued to emerge. Despite the higher error rates of these technologies
Sequence_assembly
Discipline in genetics
technology used. Third generation sequencing technologies such as PacBio or Oxford Nanopore routinely generate sequencing reads 10-100 kb in length; however
Genomics
Defective viral particles
PMID 23514891. Jaworski E, Routh A (May 2017). "Parallel ClickSeq and Nanopore sequencing elucidates the rapid evolution of defective-interfering RNAs in Flock
Defective interfering particle
Defective_interfering_particle
Chemical compound
Ridder, Jeroen (2025-06-05). "Direct detection of 8-oxo-dG using nanopore sequencing". Nature Communications. 16 (1): 5236. doi:10.1038/s41467-025-60391-3
8-Oxo-2'-deoxyguanosine
Species of virus
data was obtained from the Hungarian samples in 2020, using the Nanopore sequencing technique. The infectious virus was isolated from Schreibers's long-fingered
Lloviu_virus
Synthetic DNA analog experiment
compared to the chemical method. It also describes a way to apply nanopore sequencing to 12-base DNA. In a 2024 article from the same laboratory, the concept
Artificially Expanded Genetic Information System
Artificially_Expanded_Genetic_Information_System
Field of study
the ability to collect genome-wide epigenetic data in bacteria. Nanopore sequencing is based on changes of electrolytic current signals according to
Epigenomics
Open-Source Workflow Management Platform
Fast, and Robust Workflow for SARS-CoV-2 Genome Reconstruction via Nanopore Sequencing". Frontiers in Genetics. 12 711437. doi:10.3389/fgene.2021.711437
Nextflow
Single-molecule sequencing technology
Transmission electron microscopy DNA sequencing is a single-molecule sequencing technology that uses transmission electron microscopy techniques. The method
Transmission electron microscopy DNA sequencing
Transmission_electron_microscopy_DNA_sequencing
Repeating sequences of 2–13 base pairs of DNA
Weymaere J, Rubben K, Deforce D, Van Nieuwerburgh F (April 2020). "Nanopore Sequencing of a Forensic STR Multiplex Reveals Loci Suitable for Single-Contributor
Microsatellite
Species of beetle
Jach, Monika Elżbieta; Wiater, Adrian (2022-03-15). "Comparative Nanopore Sequencing-Based Evaluation of the Midgut Microbiota of the Summer Chafer (Amphimallon
Amphimallon_solstitiale
British biochemist
Wiggin, M.; Schloss, J. A. (2008). "The potential and challenges of nanopore sequencing". Nature Biotechnology. 26 (10): 1146–1153. doi:10.1038/nbt.1495
Hagan_Bayley
American physicist (1946–2018)
science to achieve very rapid sequencing of the entire human genome. Most recently he was involved with the Oxford Nanopore Group as it seeks to develop
Jene_Golovchenko
Genetic technique for identifying organisms in mixed samples
single species. Emerging technologies such as nanopore sequencing have resulted in the cost of DNA sequencing reducing from about USD 30,000 per megabyte
Metabarcoding
Family of proteins
beta-barrel. MspA has biotechnological applications, most notably in nanopore sequencing. Niederweis M, Danilchanka O, Huff J, Hoffmann C, Engelhardt H (2010)
Mycobacterial_porin
Electrochemistry: Nanopore beyond Sequencing | HKUST Jockey Club Institute for Advanced Study". ias.hkust.edu.hk. Retrieved 2026-06-04. "Nanopore Electrochemistry:
Nanoelectrochemistry
mechanistic interactions between pathogens, microbes, and their hosts. Nanopore sequencing - Profiling organelle genomes in the holobiont C. ashmeadii revealed
Hologenomics
American prize for basic research
Donna Strickland 2023 Sketch to Concept: Unraveling the Invention of Nanopore Sequencing David Deamer, Mark Akeson, and Daniel Branton Agrobacterium: Nature’s
Golden_Goose_Award
net income of £38 million. Oxford Nanopore Technologies – is a company that develops and sells nanopore sequencing products for the direct, electronic
List of companies of the United Kingdom K–Z
List_of_companies_of_the_United_Kingdom_K–Z
American chemist
known for expanding the studies on nanopore technology by developing a method for detecting DNA damage using a nanopore., One of the objectives of the Burrows
Cynthia_Burrows
American professor of medicine
News. In 2022 Ashley and his research team developed ultra-rapid nanopore genome sequencing for critically ill patients. Published in the New England Journal
Euan_Ashley
American genomics expert
Her research efforts make use of long-read sequencing strategies. She makes use of the Oxford Nanopore Technologies MinION sequencer, which analyses
Karen_Miga
Topics referred to by the same term
also Russian-born American singer. Genia Technologies, a US nanopore based DNA sequencing company, a fully owned subsidiary of Hoffmann-La Roche Will
Genia
Winston (April 2017). "Detecting DNA cytosine methylation using nanopore sequencing". Nature Methods. 14 (4): 407–410. doi:10.1038/nmeth.4184. PMID 28218898
Read_(biology)
American cell biologist (born 1932)
composition of the cell membrane and later in the development of DNA nanopore sequencing. Branton earned a Bachelor of Arts in mathematics at Cornell University
Daniel_Branton
Study of genes found in the environment
ecosystems. Metagenomic studies most commonly employ shotgun sequencing though long-read sequencing is being increasingly utilised as technologies advance.
Metagenomics
Digital nucleic acid sequence database
"Scaffolding and completing genome assemblies in real-time with nanopore sequencing". Nature Communications. 8 (1) 14515. Bibcode:2017NatCo...814515C
Reference_genome
Toxin found in earthworms
decades of studies into finding suitable pores for converting into nanopore sequencing systems that can have their conductive properties tuned by point
Lysenin
Topics referred to by the same term
Minion (solver), constraint solver MinION, a nanopore DNA sequencing platform developed by Oxford Nanopore Technologies Minion (typeface), a typeface designed
Minion
Species of bacteria
Digital DNA-DNA Hybridization Analysis Following PromethION Nanopore-Based Whole Genome Sequencing Allows for Accurate Prokaryotic Typing". Diagnostics. 14
Myxococcus llanfairpwllgwyngyllgogerychwyrndrobwllllantysiliogogogochensis
Myxococcus_llanfairpwllgwyngyllgogerychwyrndrobwllllantysiliogogogochensis
Species of roundworm
read sequencing project showed the diversity found in a single culture line of worms and demonstrated some methods to correct for nanopore sequencing errors
Nippostrongylus_brasiliensis
Software in bioinformatics
technologies like PacBio and fourth generation technologies like Oxford Nanopore (called long read technologies) are longer with read lengths typically
De_novo_sequence_assemblers
Public research institution in California
scientists and engineers work on a variety of projects related to genome sequencing, computational biology, large data analytics, and data sharing. The institute
UC Santa Cruz Genomics Institute
UC_Santa_Cruz_Genomics_Institute
American physician
SOLiD, Oxford Nanopore Among Others". PR Newswire (Press release). March 28, 2024. Retrieved July 18, 2024. "Whole Genome And Exome Sequencing Markets, Q4
Brandon_Colby
DNA sequencing method
Tilgner, Hagen U. (2022-04-01). "Sequencing of individual barcoded cDNAs using Pacific Biosciences and Oxford Nanopore Technologies reveals platform-specific
Circular_consensus_sequencing
Overview of and topical guide to nanotechnology
Self-replication – Micromachinery – Nano-abacus – Nanomotor – Nanopore – Nanopore sequencing – Quantum point contact – Synthetic molecular motors – Carbon
Outline_of_nanotechnology
Intersection of the fields of evolution and genomics
"Next-generation fungal identification using target enrichment and Nanopore sequencing". BMC Genomics. 24 (1): 581. doi:10.1186/s12864-023-09691-w. PMC 10544392
Phylogenomics
Website and app for sharing biodiversity observations
Lab- Early Lessons and Contributions from a New Community-Driven Nanopore Sequencing Laboratory". ResearchGate.net. January 2024. FNPS Magnolia Chapter
INaturalist
Technology for examining cells
Typically, bulk DNA methylation analysis uses bisulfite conversion or nanopore sequencing technologies to measure methylation. Some of these analyses have
Spatial DNA methylation profiling
Spatial_DNA_methylation_profiling
American scientist (born 1964)
Device for Identifying Translocating Charge-Tagged Molecules in A Nanopore Sequencing Device", published 2013-03-21, issued 2015-07-28 Jaramillo-Botero
Andres_Jaramillo-Botero
PMID 26408523. Jaworski, E; Routh, A (May 2017). "Parallel ClickSeq and Nanopore sequencing elucidates the rapid evolution of defective-interfering RNAs in Flock
ClickSeq
Physicist and engineer
bio-molecular sensing. He proposed and developed semiconductor nanopore technologies for DNA sequencing and biosensing applications, leveraging solid-state membranes
Jean-Pierre_Leburton
File format for sequences and quality scores
assign a placeholder base quality (Q0) to all bases in the read. Oxford Nanopore Duplex reads, called using the dorado basecaller are typically stored in
FASTQ_format
British virologist
Loman developed an interest in emerging viral infections, using Nanopore sequencing to track the spread of Ebola in the Western African Ebola virus epidemic
Nick_Loman
Modification of the polymerase chain reaction
in conjunction with Oxford Nanopore devices has proven to be an efficient method for the full length 16S rRNA gene sequencing for microbial community deconvolution
Reverse complement polymerase chain reaction
Reverse_complement_polymerase_chain_reaction
Species of bacterium
Shibayama K, Suzuki M, Hoshino Y (May 2018). "T, Obtained Using Nanopore and Illumina Sequencing Technologies". Genome Announcements. 6 (20). doi:10.1128/genomeA
Mycobacterium_marinum
Chemical compound
; Marchand, Jorge A. (26 October 2023). "Enzymatic synthesis and nanopore sequencing of 12-letter supernumerary DNA". Nature Communications. 14 (1) 6820
2,6-Diaminopurine
Suite of free genome analysis software by Nvidia
"Accelerated identification of disease-causing variants with ultra-rapid nanopore genome sequencing". Nature Biotechnology. 40 (7): 1035–1041. doi:10.1038/s41587-022-01221-5
Nvidia_Parabricks
possible application of nanotechnology in human genome sequencing (e.g. nanopores-based sequencing) also raises similar ethical and societal concerns. European
Ethics_of_nanotechnologies
studies (see also Transcriptomics technologies) based on next-generation sequencing technologies. This technique is largely dependent on bioinformatics tools
List of RNA-Seq bioinformatics tools
List_of_RNA-Seq_bioinformatics_tools
German physicist (born 1961)
bio-research he has pioneered nanopore sequencing technology. In 2008 his group demonstrated that a mutated version of the biological nanopore MspA could pass DNA
Jens_H._Gundlach
Nucleosome occupancy and methylome technique
adapted from NOMe-seq that uses nanopore sequencing instead of bisulfite sequencing. Nanopore sequencing is a long read sequencing method that also detects DNA
NOMe-seq
chromatin accessibility and methylation on human cell lines with nanopore sequencing". Nature Methods. 17 (12): 1191–1199. bioRxiv 10.1101/504993. doi:10
List of single cell omics methods
List_of_single_cell_omics_methods
Species of yeast
first complete genome sequencing of T. austroamericanum (type strain CBS 17435) was performed using long-read Nanopore sequencing technology. Average Nucleotide
Trichosporon_austroamericanum
American biologist and geneticist
University of California, Santa Cruz in 2015. She uses nanopore sequencing for full length RNA sequencing. In 2016 she was awarded a grant from the Santa Cruz
Angela_N._Brooks
Biophysicist
passed through it, that was used later by Oxford Nanopore technologies for DNA nanopore sequencing). His research was strongly based on the study of
Oleg Vladimirovich Krasilnikov
Oleg_Vladimirovich_Krasilnikov
Academic journal
published Title citation 2022 Kono, N. & Arakawa, K. 61 316- 326 2019 Nanopore sequencing: Review of potential applications in functional genomics 54 2021
Development, Growth & Differentiation
Development,_Growth_&_Differentiation
Genetic mutation not inherited from a parent
Oxford Nanopore and PacBio. Some types of next generation sequencing is whole genome sequencing, whole exome sequencing and deep sequencing. Whole genome
De_novo_mutation
Species of flowering plant
(2020-02-01). "Chromosomal-level assembly of Juglans sigillata genome using Nanopore, BioNano, and Hi-C analysis". GigaScience. 9 (2). doi:10.1093/gigascience/giaa006
Juglans_sigillata
high-resolution mass spectrometer. Another suggested approach has been to use nanopore sequencing technology, although questions of whether the solar radiation experienced
Polyelectrolyte theory of the gene
Polyelectrolyte_theory_of_the_gene
American scientist
control the shape of nanopores, indicating it may be possible to fabricate them using light. By removing individual atoms from the nanopores using ion beams
Marija_Drndic
Genome assembly algorithm
large genomes projects. SPAdes works with Ion Torrent, PacBio, Oxford Nanopore, and Illumina paired-end, mate-pairs and single reads. SPAdes has been
SPAdes_(software)
Brazilian scientist (born 1990)
Loman at the University of Birmingham, where she was trained in nanopore sequencing. After earning her doctoral degree Góes de Jesus joined the University
Jaqueline_Goés_de_Jesus
British-American physicist and biophysicist
amino acids at the single-molecule level. He also contributed to nanopore sequencing technologies as co-author of a 2008 Nature Biotechnology review.
Stuart_M._Lindsay
(HiFi) and Oxford Nanopore Technologies (ONT) long-read sequencing, Bionano optical maps and high-coverage Hi-C Illumina short-read sequencing. These assemblies
Human_Pangenome_Reference
increase costs per assay to the consumer. Notably, while promising, nanopore sequencing is (i) still quite early in development; and (ii) remains unproven
Top-down_proteomics
South Korean genetics project
version of KOREF was then constructed using long-read sequencing data produced by Oxford Nanopore PromethION and PacBio technologies has been released
Korean_Genome_Project
Research Institute at the University of Oxford
sequencing (HTS) services, using Illumina HiSeq4000 2500 and NextSeq500 and MiSeq. They also offer Oxford Nanopore MinION and PromethION sequencing.
Wellcome Centre for Human Genetics
Wellcome_Centre_for_Human_Genetics
Chemical compound
Oxford Nanopore added a hydroxymethyl cytosine detection model to their research basecaller, rerio, allowing old signal-level data from any R9+ nanopore runs
5-Hydroxymethylcytosine
British technology investment company
Nanopore Technologies is a life sciences company that develops nanopore‑based sequencing technologies for the analysis of DNA and RNA. The company completed
IP_Group
Life science research institute in Norwich, England
MiSeq Illumina iSeq100 PacBio Sequel PacBio Sequel II Oxford Nanopore MinION Oxford Nanopore GridION 10X Chromium Scientists at Earlham Institute and Quadram
Earlham_Institute
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