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Type of DNA library
A cDNA library is a combination of cloned cDNA (complementary DNA) fragments inserted into a collection of host cells, which constitute some portion of
CDNA_library
DNA reverse transcribed from RNA
complementary DNA (cDNA) is DNA that was reverse transcribed (via reverse transcriptase) from an RNA (e.g., messenger RNA or microRNA). cDNA exists in both
Complementary_DNA
Molecular biology technique
amplifying a library of such in bacterial plasmids. In 1982–1983, the idea of selecting random or semi-random clones from such a cDNA library for sequencing
Serial analysis of gene expression
Serial_analysis_of_gene_expression
Collection of genetic material fragments
There are different types of DNA libraries, including cDNA libraries (formed from reverse-transcribed RNA), genomic libraries (formed from genomic DNA) and
Library_(biology)
Brand of data center GPUs by AMD
the CDNA 3 architecture, which is optimized for high-performance computing (HPC) and generative artificial intelligence (AI) workloads. The CDNA 3 architecture
AMD_Instinct
Protein-coding gene in the species Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
TRAPPC4
Sub-sequence DNA in genetics
one-shot sequencing of a cloned cDNA. The cDNAs used for EST generation are typically individual clones from a cDNA library. The resulting sequence is a
Expressed_sequence_tag
Lock-and-key pairing between two structures
hybrids between RNA and DNA, and opens the door to modern tools such as cDNA libraries. While most complementarity is seen between two separate strings of
Complementarity (molecular biology)
Complementarity_(molecular_biology)
Sequencing technique for non-coding RNA
templates, to generate cDNA products for next-generation sequencing (NGS). Overall, OTTR offers a streamlined approach for cDNA library production of full-length
Ordered_two-template_relay
to a company associated with Open Biosystems. complementary DNA (cDNA) cDNA library expressed sequence tags (EST) vector (molecular biology) cloning vector
IMAGE_cDNA_clones
Protein-coding gene in the species Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
ZNF274
To identify peptides binding a target
peptide library in which each peptide is covalently linked to an mRNA:cDNA which encodes it. The immobilized target is mixed with the library in a binding
MRNA_display
Protein family
kinase refers to the first cloning of a SGK family member from a cDNA library screen for genes upregulated by the glucocorticoid dexamethasone in a
SGK
Protein-coding gene in the species Homo sapiens
1994). "Isolation of novel and known genes from a human fetal cochlear cDNA library using subtractive hybridization and differential screening". Genomics
SEMA3C
isolated RNA molecules from a tester sample to samples in a control cDNA library. It is a variant of the northern blot in which the nucleic acid immobilized
Reverse_northern_blot
Lab technique in cellular biology
at a specific time. It enables transcriptome-wide analysis by sequencing cDNA derived from RNA. Modern workflows often incorporate pseudoalignment tools
RNA-Seq
Parasitic disease caused by a family of nematode worms
to screen against the W.bancrofti Larval stage 3 cDNA library. This led to the identification of a cDNA sequence of W.bancrofti SXP-1 that encoded for a
Filariasis
Protein-coding gene in humans
(September 1994). "The addition of 5'-coding information to a 3'-directed cDNA library improves analysis of gene expression". Gene. 146 (2): 199–207. doi:10
CHD1L
Protein-coding gene in humans
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
CLDN5
Protein-coding gene in the species Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
Mitochondrial ribosomal protein L24
Mitochondrial_ribosomal_protein_L24
Protein-coding gene in the species Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
ACSM3
Technology for examining cells
well as the simultaneous generation of a cDNA library from mRNAs. Next, spatial barcodes are added to the cDNA and gDNA to capture the location of these
Spatial DNA methylation profiling
Spatial_DNA_methylation_profiling
Process of isolation and amplification of DNA segments
obtain the cDNA for a specific gene, it is first necessary to construct a cDNA library. The DNA of interest needs to be fragmented to provide a relevant DNA
In_vitro_recombination
Protein-coding gene in the species Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–156. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
DNAL4
Protein-coding gene in humans
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
ZNF451
Range of methods designed for assigning cell types
in the film. This method allows further RNA transcript profiling and cDNA library generation of the retrieved cells. RNA sequencing of the selected regions
Spatial_transcriptomics
Protein involved in the breakdown of blood clots
immunoprecipitation. The resulting cDNA library was subsequently screened via sequence analysis and compared to a whole genome library for confirmation of specific
Tissue-type plasminogen activator
Tissue-type_plasminogen_activator
American biologist
full length double stranded DNA copies of messenger RNA (termed “copy” or cDNA). This method provided a key step in the isolation of human genes, and in
Tom_Maniatis
Protein-coding gene in the species Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
PDCD6
Protein-coding gene in the species Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
GINS2
Protein-coding gene in the species Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
MTCH2
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
Secretoneurin
Mammalian protein found in Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
Uroporphyrinogen III decarboxylase
Uroporphyrinogen_III_decarboxylase
Protein-coding gene in humans
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
MAGEA3
Type of enzyme
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–156. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
Lactate_dehydrogenase_A
Protein-coding gene in the species Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
EIF3EIP
Disease causing insufficient blood cells of all types
the serologic screening of a fetal liver library with sera from 8 patients. The human fetal liver cDNA library (chosen because of its high enrichment of
Aplastic_anemia
Protein-coding gene in the species Homo sapiens
type. Fast skeletal muscle TnI was first cloned from a skeletal muscle cDNA library. It is generally observed that fsTnI is exclusively expressed in fast
Troponin I, fast skeletal muscle
Troponin_I,_fast_skeletal_muscle
Protein-coding gene in the species Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
ELP3
Protein-coding gene in the species Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
Discoidin domain-containing receptor 2
Discoidin_domain-containing_receptor_2
Protein-coding gene in the species Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
NPDC1
Protein-coding gene in the species Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
EIF4G3
Medical condition
about 8kb with 13 exon sequences. It was successfully cloned from a cDNA library in 1995 revealing that, after processing, it is 477 nucleotides long
Variegate_porphyria
Mammalian protein found in Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
FCGR2A
Ability of a cell to take up DNA
Cells for High-Efficiency Plasmid Transformation of Escherichia coli". CDNA Library Protocols. Methods in Molecular Biology. Vol. 69. pp. 129–138. doi:10
Transformation_efficiency
Enzyme which generates DNA
convert single-stranded RNA into double-stranded cDNA. In retroviruses and retrotransposons, this cDNA can then be endogenized into the host genome, from
Reverse_transcriptase
Protein-coding gene in the species Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
EIF3H
Enzyme of the glycolysis metabolic pathway
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
GAPDHS
Protein-coding gene in the species Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
SMTN
Nuclear protein inhibiting DNA replication
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–156. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
Geminin
Protein-coding gene in the species Homo sapiens
5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149. IFNGR1+protein,+human at the U.S. National Library of Medicine
Interferon_gamma_receptor_1
high-salt wash, SDS-PAGE, membrane transfer, proteinase digestion, cDNA library preparation and sequencing in order to identify the direct RNA binding
RNA_immunoprecipitation_chip
Protein-coding gene in the species Homo sapiens
Watanabe S, Suzuki F, Yamaguchi N (Oct 1992). "Use of an epitope-tagged cDNA library to isolate cDNAs encoding proteins with nuclear localization potential"
PPIC
Protein domain
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
GMP_synthase
Protein-coding gene in the species Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
HDHD1A
Protein-coding gene in humans
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–156. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
COPS4
nucleotides at the end) to create a cDNA library for each sample, followed by PCR amplification using arbitrary 3′ primers for cDNA strand amplification together
Differential_display
Protein-coding gene in the species Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
Acylglycerol kinase, mitochondrial
Acylglycerol_kinase,_mitochondrial
Hierarchical outline list of articles related to genetics
bone marrow transplantation cancer candidate gene carcinoma carrier cDNA library cell centimorgan centromere chromosome chromosomal translocation cloning
Outline_of_genetics
Protein-coding gene in the species Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–156. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
SH2D4A
Protein-coding gene in the species Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
CRHBP
Protein-coding gene in the species Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–156. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
GAS7
Protein-coding gene in the species Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
STRN4
Protein-coding gene in the species Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–156. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
TREM1
Protein-coding gene in the species Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–156. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
TRIM21
Protein and coding gene in humans
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–156. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
HOMER1
Protein-coding gene in the species Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
DEPTOR
Protein-coding gene in the species Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
DAP3
assay, a genomic or cDNA library is screened to identify the genetic sequence of a protein of interest. Expression cDNA libraries may be screened with
Functional_cloning
Protein-coding gene in humans
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–156. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
CDK13
Protein-coding gene in humans
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
CCNDBP1
Protein-coding gene in the species Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
SART3
Protein-coding gene in the species Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
ME1_(gene)
Protein-coding gene in the species Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–156. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
Glypican_1
Protein-coding gene in the species Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
GTF2H4
Protein-coding gene in the species Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
FLOT1
Protein-coding gene in the species Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
SGK3
Protein-coding gene in the species Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
SARDH
Protein-coding gene in the species Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–156. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
SUMO2
Protein-coding gene in the species Homo sapiens
"Identification of 4370 expressed sequence tags from a 3'-end-specific cDNA library of human skeletal muscle by DNA sequencing and filter hybridization"
Filamin-C
sequencing facility, where it is first reverse transcribed to create a cDNA library. This cDNA can then be fragmented into various lengths depending on the platform
De novo transcriptome assembly
De_novo_transcriptome_assembly
Protein-coding gene in the species Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
NLRP2
Protein-coding gene in the species Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
DGKZ
Protein-coding gene in the species Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
FBXO11
Protein-coding gene in the species Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
INTS9
Protein-coding gene in the species Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
SERPINB1
Protein-coding gene in the species Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
PEX6
transcriptional repression. BAHD1 was first cloned from a human brain cDNA library and the coding sequence was named KIAA0945. Bierne and colleagues further
Bromo adjacent homology domain containing 1
Bromo_adjacent_homology_domain_containing_1
Protein-coding gene in the species Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
SNX2
Protein-coding gene in the species Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
Monocarboxylate_transporter_5
Method used in molecular biology
sites that contained the expected Nova-binding motifs. Sequencing of the cDNA library identified many positions close to alternative exons, several of which
Cross-linking immunoprecipitation
Cross-linking_immunoprecipitation
Protein-coding gene in humans
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
WAC_(gene)
Protein-coding gene
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
TMED10
Protein-coding gene in the species Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
RFX5
Enzyme found in humans
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
PSMD12
Protein-coding gene in the species Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
DR1_(gene)
Protein-coding gene in the species Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
HSF2
Protein-coding gene in the species Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
LRRC8D
Protein-coding gene in the species Homo sapiens
and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149
HUWE1
Set of all RNA molecules in one cell or a population of cells
and proteome. The first study to present a case of a collection of a cDNA library for silk moth mRNA was published in 1979. The first seminal study to
Transcriptome
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