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Class of enzymes which cleave nucleic acids
In biochemistry, a nuclease (also archaically known as nucleodepolymerase or polynucleotidase) is an enzyme capable of cleaving the phosphodiester bonds
Nuclease
Artificial enzymes
Zinc-finger nucleases (ZFNs) are artificial restriction enzymes generated by fusing a zinc finger DNA-binding domain to a DNA-cleavage domain. Zinc finger-domains
Zinc-finger_nuclease
Type of genetic engineering
of engineered nucleases were used: meganucleases, zinc finger nucleases (ZFNs), transcription activator-like effector-based nucleases (TALEN). All of
Genome_editing
Class of enzymes
Serratia marcescens nuclease (EC 3.1.30.2, endonuclease (Serratia marcescens), barley nuclease, plant nuclease I, nucleate endonuclease) is an enzyme.
Serratia_marcescens_nuclease
Crab duplex-specific nuclease is a nuclease derived from the red king crab (Paralithodes camtschaticus, Kamchatka crab) hepatopancreas that displays a
Crab_duplex-specific_nuclease
Chimeric nucleases are an example of engineered proteins which must comprise a DNA-binding domain to give sequence specificity and a nuclease domain for
Chimeric_nuclease
Class of enzymes
Micrococcal nuclease (EC 3.1.31.1, S7 Nuclease, MNase, spleen endonuclease, thermonuclease, nuclease T, micrococcal endonuclease, nuclease T', staphylococcal
Micrococcal_nuclease
Enzyme used to distinguish between single and double stranded DNA and RNA
Mung bean nuclease (Nuclease MB) is a nuclease derived from sprouts of the mung bean (Vigna radiata) that removes nucleotides in a step-wise manner from
Mung_bean_nuclease
Enzymes that cleave DNA in specific ways
Transcription activator-like effector nucleases (TALEN) are restriction enzymes that can be engineered to cut specific sequences of DNA. They are made
Transcription activator-like effector nuclease
Transcription_activator-like_effector_nuclease
Surveyor nuclease assay is an enzyme mismatch cleavage assay used to detect single base mismatches or small insertions or deletions (indels). Surveyor
Surveyor_nuclease_assay
Class of enzymes
Nuclease S1 (EC 3.1.30.1) is an endonuclease enzyme that splits single-stranded DNA (ssDNA) and RNA into oligo- or mononucleotides. This enzyme catalyses
Nuclease_S1
Type of unintended effects of genetic modification techniques
unintended genetic modifications that can arise through the use of engineered nuclease technologies such as: clustered, regularly interspaced, short palindromic
Off-target_genome_editing
Class of enzymes that divide DNA
be generated by fusing a natural or engineered DNA-binding domain to a nuclease domain (often the cleavage domain of the type IIS restriction enzyme FokI)
Restriction_enzyme
Laboratory technique used in biochemistry and genetics
Nuclease protection assay is a laboratory technique used in biochemistry and genetics to identify individual RNA molecules in a heterogeneous RNA sample
Nuclease_protection_assay
Protein-coding gene in the species Homo sapiens
Information, U.S. National Library of Medicine. "Entrez Gene: APEX1 APEX nuclease (multifunctional DNA repair enzyme) 1". Parker A, Gu Y, Mahoney W, Lee
APEX1
Microbial protein found in Streptococcus pyogenes M1 GAS
between Cas9 and sgRNA. Cas9 contains two nuclease domains an McrA-like HNH nuclease domain and a RuvC-like nuclease domain. These HNH domain cleaves the complementary
Cas9
Protein used in CRISPR
3′-to-5′ from the helicase domain to the nuclease domain where it is hydrolysed. Cas3 consists of an HD-nuclease domain fused to two RecA-like domains.
Cas3
RNA transcript from the CRISPR locus
Cas nuclease or single effector protein will cause interference guided by the crRNA match. Type-I CRISPR systems are characterized by Cas3, a nuclease-helicase
CRISPR_RNA
Small structural protein motif found mostly in transcriptional proteins
for a specific sequence is an area of active research, and zinc finger nucleases and zinc finger transcription factors are two of the most important applications
Zinc_finger
Restriction enzyme
helices α4 and α5 and two loops P1 and P2 of the cleavage domain. When the nuclease is unbound to DNA, the endonuclease domain is sequestered by the DNA-binding
FokI
Experimental gene therapy
the two zinc finger nuclease enables specific DNA targeting. Specificity of genome editing is important for the zinc finger nuclease to be a successful
Zinc finger nuclease treatment of HIV
Zinc_finger_nuclease_treatment_of_HIV
Enzyme
in lysosomes, which are acidic on the inside. Acid hydrolases may be nucleases, proteases, glycosidases, lipases, phosphatases, sulfatases and phospholipases
Acid_hydrolase
Method used to analyze protein interactions with DNA
CUT&RUN sequencing, also known as cleavage under targets and release using nuclease, is a method used to analyze protein interactions with DNA. CUT&RUN sequencing
CUT&RUN_sequencing
with the dual ligation assay. The nuclease hybridization assay, also called S1 nuclease cutting assay, is a nuclease protection assay-based hybridization
Hybridization_assay
issues in higher complex organisms. Transcription activator-like effector nuclease, TALENs, are a set of restriction enzymes that be created to cut out desired
Insert_(molecular_biology)
South Korean biochemist (born 1964)
programmable nucleases, specifically zinc finger nucleases (ZFNs), TAL effector nucleases (TALENs), and RNA-guided engineered nucleases (RGENs). In 2018
Kim_Jin-soo_(biologist)
Protein used in CRISPR
cysteine residues to bind an iron-sulfer cluster like those of the AddB nuclease of Bacillus subtilis. The Cas4 family protein Sso0001 codes is single stranded
Cas4
Family of DNA sequences found in prokaryotic organisms
CRISPR-associated systems, Cas 1–4. The Cas proteins showed helicase and nuclease motifs, suggesting a role in the dynamic structure of the CRISPR loci.
CRISPR
Type of base-pair DNA sequence
DNA sequence immediately following the DNA sequence targeted by the Cas9 nuclease in the CRISPR bacterial adaptive immune system. The PAM is a component
Protospacer_adjacent_motif
Enzymes which cleave a nucleotide chain
enzymes known as "exo-endonucleases", however, are not limited to either nuclease function, displaying qualities that are both endo- and exo-like. Evidence
Endonuclease
German biologist
diseases. In 2023, his work led to the first characterization of the Cas12a2 nuclease, which represents a completely new type of gene scissors. The discovery
Chase_Beisel
Topics referred to by the same term
Examples of gene editing are CRISPR, zinc finger nuclease, transcription activator-like effector nuclease (TALEN), oligonucleotide directed mutagenesis +
Gene_editing
Protein-coding gene in the species Homo sapiens
FANCD2/FANCI-associated nuclease 1 (KIAA1018) is an enzyme that in humans is encoded by the FAN1 gene. It is a structure dependent endonuclease. It is
FAN1
Protein-coding gene in the species Homo sapiens
(PARN), also known as polyadenylate-specific ribonuclease or deadenylating nuclease (DAN), is an enzyme that in humans is encoded by the PARN gene. Exonucleolytic
Poly(A)-specific_ribonuclease
a large discovery, as Rad10 associated with Rad1 proteins to create a nuclease within NER pathways. In additional studies conducted by Zhu et al. (2003)
Excision repair cross-complementing
Excision_repair_cross-complementing
Measurement of genetic variations
needed] A broad range of enzymes including DNA ligase, DNA polymerase and nucleases have been employed to generate high-fidelity SNP genotyping methods. Restriction
SNP_genotyping
Class of enzyme that catalyzes the degradation of RNA
Ribonuclease (commonly abbreviated RNase) is a type of nuclease that catalyzes the degradation of RNA into smaller components. Ribonucleases can be divided
Ribonuclease
Ribonuclease III enzyme
humans is encoded by the DROSHA (formerly RNASEN) gene. It is the primary nuclease that executes the initiation step of miRNA processing in the nucleus. It
Drosha
Lebanese biotechnologist (born 1965)
André Choulika (born 1965) is a biotechnologist, the inventor of nuclease-based genome editing and a pioneer in the analysis and use of meganucleases to
André_Choulika
Genetic technique
gene. Transcription activator-like effector nucleases (TALENs) also contain a DNA binding domain and a nuclease that can cleave DNA. The DNA binding region
Gene_knockout
Class of enzymes
carbohydrates such as starch and sugars into simple sugars such as glucose, and nucleases split nucleic acids into nucleotides. Digestive enzymes are found throughout
Digestive_enzyme
Protein-coding gene in the species Homo sapiens
enzyme complex that participates in DNA repair and DNA recombination. The nuclease enzyme ERCC1-XPF cuts specific structures of DNA. Many aspects of these
ERCC4
on the gel. Primer extension offers an alternative to a nuclease protection assay (S1 nuclease mapping) for quantifying and mapping RNA transcripts. The
Primer_extension
Method used to analyse protein interactions with DNA
MNase-seq, short for micrococcal nuclease digestion with deep sequencing, is a molecular biological technique that was first pioneered in 2006 to measure
MNase-seq
Gene editing method
bacterial CRISPR-Cas9 antiviral defense system. By delivering the Cas9 nuclease complexed with a synthetic guide RNA (gRNA) into a cell, the cell's genome
CRISPR_gene_editing
Protein manipulation product
The CompoZr Zinc finger nuclease (ZFN) platform is a technology developed by Sigma-Aldrich that allows researchers to target and manipulate the genome
CompoZr
and open the unfinished DNA using enzyme nucleases such as SLX1-SLX4 and MUS81-EME1. Typically, these nucleases will work as the cell transitions from the
Mitotic_DNA_Synthesis
Type of genetically engineered rat
Norway rat (Rattus norvegicus). Additional developments with zinc finger nuclease technology in 2009 led to the first knockout rat with targeted, germline-transmitted
Knockout_rat
Protein-coding gene in the species Homo sapiens
binding protein 1 (YB-1), also known as Y-box transcription factor or nuclease-sensitive element-binding protein 1 is a protein that in humans is encoded
Y_box_binding_protein_1
American professor of medicine
first to demonstrate precise gene editing in human cells using chimeric nucleases. He graduated magna cum laude from Harvard University and completed his
Matthew_Porteus
Ribonuclease M5 (EC 3.1.26.8, RNase M5, 5S ribosomal maturation nuclease, 5S ribosomal RNA maturation endonuclease) is an enzyme. This enzyme catalyses
Ribonuclease_M5
very uniform tiny crystals for controlled dosage. 1971 – Staphylococcal nuclease 1971 – Cytochrome C 1974 – T4 phage lysozyme 1974 – Immunoglobulins 1975 –
List of biophysically important macromolecular crystal structures
List_of_biophysically_important_macromolecular_crystal_structures
Molecule that carries genetic information
are most accessible. Nucleases are enzymes that cut DNA strands by catalyzing the hydrolysis of the phosphodiester bonds. Nucleases that hydrolyse nucleotides
DNA
Human gene
BioGPS More reference expression data Gene ontology Molecular function nuclease activity exonuclease activity hydrolase activity RNA binding nucleic acid
RNA_exonuclease_5
Genetic disorder
currently conducting a clinical trial involving gene editing using zinc finger nuclease (ZFN) for the treatment of MPS I. In 1919, Gertrud Hurler, a German pediatrician
Hurler_syndrome
DNA researcher and scientist
School of Public Health. He is credited for the creation of zinc finger nucleases (ZFN) technology, which is capable of cleaving the structure of DNA. Professor
Srinivasan_Chandrasegaran
Ribonuclease enzyme found in the venom of the Caspian cobra
retroviruses, such as hepatitis C, dengue virus, and HIV. Together with S1 nuclease, which specifically cleaves single-stranded RNA, it can be used to profile
Ribonuclease_V1
Protein-coding gene in the species Homo sapiens
endonuclease of the DNase family coded by the human gene DNASE1. DNase I is a nuclease that cleaves DNA preferentially at phosphodiester linkages adjacent to
Deoxyribonuclease_I
American cell and gene therapy company
2019[update] clinical trials by Sangamo involving gene editing using zinc finger nuclease were ongoing. Sangamo's programs are a mix of wholly owned and partnered;
Sangamo_Therapeutics
Chemical compound
polymerase. Deoxyadenosine triphosphate is produced from DNA by the action of nuclease P1, adenylate kinase, and pyruvate kinase. High levels of dATP can be toxic
Deoxyadenosine_triphosphate
Protein and coding gene in humans
Staphylococcal nuclease domain-containing protein 1 also known as 100 kDa coactivator or Tudor domain-containing protein 11 (TDRD11) is a protein that
SND1
Repetitive DNA with distinct base composition
conformations as shown by their response to structrural nuclease enzymes including S1, P1, and mung bean nucleases. The most complex compositionally-biased microsatellite
Satellite_DNA
Parts of lagging strand in DNA replication
'tracking; model where the nuclease moves from the 5' flap to its base to preform cleavage. The Pol δ does not process a nuclease activity to cleave the displaced
Okazaki_fragments
Protein-coding gene in the species Homo sapiens
Gene: Lactamase, beta 2". Retrieved 2015-07-07. Dominski Z (2007-03-01). "Nucleases of the metallo-beta-lactamase family and their role in DNA and RNA metabolism"
LACTB2
Short strand of RNA or DNA that serves as a starting point for DNA synthesis
a result another nuclease must be recruited to cleave the flap. This second nuclease is DNA2 nuclease, which has a helicase-nuclease activity, that cleaves
Primer_(molecular_biology)
American academic
an academic field Scientific career Institutions New York University Thesis Purification and properties of a nuclease from Serratia marcescens (1968)
Marion_Nestle
American chemist (1930–2007)
from it. In 1962 he undertook the crystal structure of the Staphylococcal nuclease enzyme, solved to 2Å resolution in 1969, published in 1971, and deposited
F._Albert_Cotton
Protein-coding gene in the species Homo sapiens
reference expression data BioGPS n/a Gene ontology Molecular function nuclease activity endonuclease activity hydrolase activity transposase activity
PiggyBac transposable element derived 5
PiggyBac_transposable_element_derived_5
Protein-coding gene in the species Homo sapiens
homolog of DNA2KL present in budding yeast, possesses both helicase and nuclease activity, with which it helps catalyze early steps in homologous recombination
DNA2L
Class of enzymes which use water to break a chemical bond
common names are typically in the form "substrate base". For example, a nuclease is a hydrolase that cleaves nucleic acids. Hydrolases are classified as
Hydrolase
Protein-coding gene in the species Homo sapiens
JJ, Shen B (December 2003). "Interaction and stimulation of human FEN-1 nuclease activities by heterogeneous nuclear ribonucleoprotein A1 in alpha-segment
Flap structure-specific endonuclease 1
Flap_structure-specific_endonuclease_1
transcriptional activator (A) or repressor (R), a methylation domain (M) or a nuclease (N). Modification of the endogenous DNA-binding zinc finger domain is the
Zinc_finger_chimera
Protein-coding gene in humans
repair. The ERCC1-XPF nuclease is an essential activity in the pathway of DNA nucleotide excision repair (NER). The ERCC1-XPF nuclease also functions in pathways
ERCC1
Species of king crab
with various activities: collagenase, protease, hyaluronidase, lipase, nuclease, etc. The complex of proteolytic enzymes of the red king crab hepatopancreas
Red_king_crab
Protein-coding gene in the species Homo sapiens
Molecular function DNA binding calcium ion binding endonuclease activity nuclease activity hydrolase activity deoxyribonuclease activity metal ion binding
DNASE1L2
HT-TALENS (HIV-targeted transcription activator-like effector nucleases) is an engineered plant protein that is a proposed cure for AIDS. The HIV genome
HT-TALENS
Protein-coding gene in the species Homo sapiens
tRNA-intron endonuclease activity molecular function nucleic acid binding nuclease activity Cellular component nucleolus nucleus cellular component Biological
TSEN15
Branched nucleic acid structure
Holliday junction resolvases fall into two families, integrases and nucleases, that are each structurally similar although their sequences are not conserved
Holliday_junction
Enzyme found in humans
endoribonuclease activity ribonuclease activity protein binding RNA binding nuclease activity ribonucleoprotein complex binding endonuclease activity hydrolase
Ribonuclease_L
mismatch of the two DNA strands, which is then cleaved by a single stranded nuclease. The products are then separated by size on several different platforms
TILLING_(molecular_biology)
Gene editing technique
certain variants, all Cas9 prime editors use a nickase. Normally, Cas9 is a nuclease, slicing DNA in half to make blunt-ended double-stranded breaks. The protein
Prime_editing
Protein-coding gene in the species Homo sapiens
reference expression data Gene ontology Molecular function DNA binding nuclease activity endonuclease activity sequence-specific DNA binding protein binding
Translin
Family of protein complexes in bacteria
is both a helicase that unwinds, or separates the strands of DNA, and a nuclease that makes single-stranded nicks in DNA. It catalyses exonucleolytic cleavage
RecBCD
Protein-coding gene in the species Homo sapiens
function hydrolase activity ribonuclease T2 activity endonuclease activity nuclease activity RNA binding ribonuclease activity endoribonuclease activity Cellular
RNASET2
Type of genetic component
retrotransposons contain genes for reverse transcriptase, RNA-binding protein, nuclease, and sometimes ribonuclease H domain but they lack the long terminal repeats
Retrotransposon
MksBEF. More recently, a variant complex called MksBEFG, which includes a nuclease subunit MksG, has been shown to function in plasmid defense. In other bacterial
SMC_protein
Protein-coding gene in humans
response to DNA damage stimulus depyrimidination negative regulation of nuclease activity metabolism DNA repair base-excision repair Sources:Amigo / QuickGO
NEIL1
Protein-coding gene in the species Homo sapiens
BioGPS n/a Gene ontology Molecular function deoxyribonuclease II activity nuclease activity endonuclease activity endodeoxyribonuclease activity hydrolase
Deoxyribonuclease_2_beta
Ability of cells to take up extracellular DNA
competent bacteria also secrete nucleases into their surroundings, and all bacteria can take up the free nucleotides these nucleases generate from environmental
Natural_competence
Protein-coding gene in the species Homo sapiens
binding U2 snRNA binding RNA binding nucleic acid binding U1 snRNA binding nuclease activity exonuclease activity hydrolase activity Cellular component cytoplasm
ISG20
Histone acetylation on the tail of histone H3
Micrococcal Nuclease sequencing (MNase-seq) is used to investigate regions that are bound by well-positioned nucleosomes. Use of the micrococcal nuclease enzyme
H3K27ac
Healthcare company
development applications. It was formed in 2008 to investigate zinc finger nuclease technology and its application for disease research models. Located in
Sigma-Aldrich
Protein involved in DNA repair
(2021). "Exploring the Structures and Functions of Macromolecular SLX4-Nuclease Complexes in Genome Stability". Frontiers in Genetics. Wilson JS, Tejera
SLX4
Species of virus
in five monocistronic mRNAs. N protein encapsidates genome RNA into a nuclease-resistant form to protect it from cellular RNAse activity. L and P protein
Chandipura_virus
Medication
it binds, protecting the RNA from being destroyed by normally present nucleases; by binding to miR-122, miravirsen removes that protection and the virus
Miravirsen
Protein-coding gene in the species Homo sapiens
BioGPS More reference expression data Gene ontology Molecular function nuclease activity hydrolase activity endonuclease activity transcription corepressor
RBBP8
Protein-coding gene in the species Homo sapiens
region provides the DNA-binding abilities for the protein as well as some nuclease activity. The transposase activity is lost due to the presence of several
SETMAR
Protein-coding gene in the species Homo sapiens
helicase activity DNA helicase activity protein binding nucleic acid binding nuclease activity endonuclease activity ATP binding hydrolase activity mRNA binding
G3BP1
Protein-coding gene in the species Homo sapiens
BioGPS More reference expression data Gene ontology Molecular function nuclease activity endonuclease activity 3'-tRNA processing endoribonuclease activity
ELAC1
Topics referred to by the same term
village in Madhya Pradesh, India Transcription activator-like effector nuclease (TALEN) Talen Energy, an energy company Royal Talens, an art supplies company
Talen
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