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Method for neuronal projection mapping
MAPseq or Multiplexed Analysis of Projections by Sequencing is a RNA-Seq based method for high-throughput mapping of neuronal projections. It was developed
MAPseq
Species of bacterium
structured RNA molecules. A method for determining RNA secondary structure, DMS-MaPseq, uses this enzyme because it converts normal RNA to DNA accurately but introduces
Geobacillus stearothermophilus
Geobacillus_stearothermophilus
Chemical compound
cannot methylate base-paired nucleotides. Using the method known as DMS-MaPseq, RNA is incubated with DMS to methylate unpaired bases. Then the RNA is
Dimethyl_sulfate
Experimental process
truncation-based methods, DMS mutational profiling with sequencing (DMS-MaPseq) can detect multiple DMS modifications in a single RNA molecule, which enables
Nucleic acid structure determination
Nucleic_acid_structure_determination
initial patient visit to the physician-guided reporting of genomic findings. MapSeq (Masively Parallel Sequencing) is an open source plugin-based Service-Oriented
Renaissance Computing Institute
Renaissance_Computing_Institute
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