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DISPASE

  • Dispase
  • Dispase is a protease which cleaves fibronectin, collagen IV, and to a lesser extent collagen I. It is found in some bacteria and can be isolated from

    Dispase

    Dispase

  • Paenibacillus polymyxa
  • Species of bacterium

    Staphylococcus aureus and Streptococcus bovis. P. polymyxa is a source of dispase, an enzyme used to isolate cells from animal tissues. Mahajan GB, Balachandran

    Paenibacillus polymyxa

    Paenibacillus_polymyxa

  • Decellularization
  • controls any potential damage it could cause on the extracellular fibers. Dispase is used to prevent undesired aggregation of cells, which is beneficial

    Decellularization

    Decellularization

    Decellularization

  • Concanavalin A
  • Lectin (carbohydrate-binding protein)

    vitreoretinopathy in rats after intraocular injection of concanavalin a and dispase]". Rossiĭskii Fiziologicheskiĭ Zhurnal Imeni I.M. Sechenova. 101 (5): 572–585

    Concanavalin A

    Concanavalin A

    Concanavalin_A

  • Proliferative vitreoretinopathy
  • Eye disease

    model of PVR and reduced the frequency of membrane formation by 43% in the dispase model of PVR and by 31% in the concanavalin one. Lornoxicam not only normalized

    Proliferative vitreoretinopathy

    Proliferative_vitreoretinopathy

  • Cyclooxygenase-2
  • Human enzyme involved in inflammation

    inflammation reduced the frequency of membrane formation by 43% in the dispase model of PVR and by 31% in the concanavalin one. Lornoxicam not only normalized

    Cyclooxygenase-2

    Cyclooxygenase-2

    Cyclooxygenase-2

  • NOMe-seq
  • Nucleosome occupancy and methylome technique

    components include Dulbecco's phosphate-buffered saline (DPBS), trypsin or dispase (depending on cell type), trypan blue, hemocytometer, lysis buffer, and

    NOMe-seq

    NOMe-seq

    NOMe-seq

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