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Class of biological molecules
Analyte-specific reagents (ASRs) are a class of biological molecules which can be used to identify and measure the amount of an individual chemical substance
Analyte-specific_reagent
Topics referred to by the same term
sensitive aggregates and causing concrete expansion and failure Analyte-specific reagent, a class of biological molecules Asian soybean rust, a plant disease
ASR
Method to detect an antigen using an antibody and enzyme
a reagent. Alternatively, if the analyte itself is an antibody, its target antigen can be used as the binding reagent. Before the development of the ELISA
ELISA
Biochemical test for a protein or other molecule using an antibody
The molecule detected by the immunoassay is often referred to as an "analyte" and is in many cases a protein, although it may be other kinds of molecules
Immunoassay
Anticancer medication
PD-L1 level as a potential biomarker using the antibody as the analyte-specific reagent. Bristol Myers Squibb partnered with Dako on a nivolumab-based
Nivolumab
Method of separating chemical or biological samples
of methods. In CE methods, analytes migrate through electrolyte solutions under the influence of an electric field. Analytes can be separated according
Capillary_electrophoresis
Measure the amount of a target entity
entity. The measured entity is often called the analyte, the measurand, or the target of the assay. The analyte can be a drug, biochemical substance, chemical
Assay
Method of determining chemical concentration
and ions. Often, analysis is completed with the aid of a reagent that reacts with the analyte to produce a colored product. Sometimes an enzymatic stage
Colorimetric_analysis
detection sensitivity, use low volumes of reagents, have short analysis times, and minimal cross-contamination of analytes, which make them efficient in many
Droplet-based_microfluidics
Method of analyzing electrochemical reactions
voltammetry is generally used to study the electrochemical properties of an analyte in solution or of a molecule that is adsorbed onto the electrode and to
Cyclic_voltammetry
Reagent for converting a chemical compound to a chiral derivative
derivatizing agent (CDA), also known as a chiral resolving reagent, is a derivatization reagent that is a chiral auxiliary used to convert a mixture of enantiomers
Chiral_derivatizing_agent
Analytical technique based on determining mass to charge ratio of ions
(CI), the analyte is ionized by a chemical reaction with an ionized reagent gas (itself ionized by some technique, such as by EI). The analyte gas and the
Mass_spectrometry
Diagnostic tool used in urinalysis
standard urine test strip may comprise up to 10 different chemical pads or reagents which react (change color) when immersed in, and then removed from, a urine
Urine_test_strip
Lowest quantity observable with sufficient confidence above the background
amount, of analyte that has a signal statistically significantly larger than the signal arising from the repeated measurements of a reagent blank. Mathematically
Detection_limit
Quantitative determination of a chemical species based on its mass
used in analytical chemistry for the quantitative determination of an analyte (the ion being analyzed) based on its mass. The principle of this type
Gravimetric_analysis
that absorbs microwave radiation, usually water. The specific blend of acids (or other reagents) and the temperatures vary depending upon the type of
Microwave_digestion
Analytical method
technique (positive CI or negative CI) chosen, this reagent gas will interact with the electrons and analyte and cause a 'soft' ionization of the molecule of
Gas chromatography–mass spectrometry
Gas_chromatography–mass_spectrometry
Type of antibody
immunoassays in which the analyte in the unknown sample is bound to the antibody site, then labeled antibody is bound to the analyte. The amount of labeled
Heterophile_antibody
Form of protein microarray
antibody microarrays would not only permit simultaneous screening of an analyte panel, but would also be more sensitive and rapid than conventional screening
Antibody_microarray
Immunochromatographic testing devices
an antibody specific to the target analyte labelled with a visual tag, usually colloidal gold. The antibodies bind to the target analyte within the sample
Lateral_flow_test
Probe which tests for biological molecules
reagentless biosensor can monitor a target analyte in a complex biological mixture without additional reagent. Therefore, it can function continuously if
Biosensor
Chemistry technique
this allows a small amount of analyte to be used. The reaction is general, allowing a wide range of substrates, yet specific to a single functional group
Derivatization
Method of transferring large biomolecules onto a carrier for analysis
matrix and specific sequences are detected with labeled RNA probes. A dot blot is a special case of any of the above blots where the analyte is added directly
Blot_(biology)
Chemical analysis used to determine quantities of substances
Analysis refers to analyses in which the amount or concentration of an analyte may be determined (estimated) and expressed as a numerical value in appropriate
Quantitative analysis (chemistry)
Quantitative_analysis_(chemistry)
Set of laboratory techniques for separation of mixtures
by the analytes exiting the system. In the case of an optimal system the signal is proportional to the concentration of the specific analyte separated
Chromatography
concentration of the analyte. This process is used in clinical diagnostics as well as food and environmental analysis due to its high specificity and ability to
Chemiluminescent_immunoassay
Analytical chemistry technique
desolvation chamber. The analytes were ionized using a solvent-assisted chemical ionization source, where the LC solvents acted as reagent gases. To use this
Liquid chromatography–mass spectrometry
Liquid_chromatography–mass_spectrometry
which enables the reactive DART reagent ion stream to flow on to the surface while allowing the flow of desorbed analyte ions into interface. Therefore
Direct_analysis_in_real_time
successful, such as volume, concentration, sample phase, and composition of the analyte solution. Quite possibly the most important consideration in sample preparation
Sample preparation in mass spectrometry
Sample_preparation_in_mass_spectrometry
Detector for separated compounds from a chromatographic column
its unique capabilities and is suitable for specific applications, depending on the nature of the analytes and the sensitivity and selectivity required
Chromatography_detector
Chemical separation technique
phase (CSP) is made of a support, usually silica based, on which a chiral reagent or a macromolecule with numerous chiral centers is bonded or immobilized
Chiral_column_chromatography
Type of chemical test in which a water sample flows through a system
again with a solution containing a signal reagent, and washed again to remove unbound signal reagent. If the analyte was present in the original sample, then
Flow-through_test
changes when it interacts with the analyte (such as a change in absorbance), the interactions between the probe and the analyte can be studied. This makes it
Molecular_probe
Medical laboratory instrument
Photometry is the most common method for testing the amount of a specific analyte in a sample. In this technique, the sample undergoes a reaction to
Automated_analyser
collisionally stabilized. The main advantage of using NH4+ reagent ions is that fragmentation of analytes upon chemical ionization is strongly suppressed, leading
Proton-transfer-reaction mass spectrometry
Proton-transfer-reaction_mass_spectrometry
Colorimetric method used in clinical chemistry
protein, from the sample and increase specificity. By the 1950s, precipitated aluminum silicate, called Lloyd's reagent, was being used to remove protein
Jaffe_reaction
Soft ionization source
processes by which it can occur. The first involved direct desorption of analyte, where evaporation of the more volatile solvent allows the less volatile
Thermospray
Compound that is derived from a similar compound by a chemical reaction
widely used. In analytical chemistry, derivatization can be used to convert analytes into other species for improving detection. For example, polar groups such
Derivative_(chemistry)
Ionization method
molecules from the protonated analyte molecule) of MH+(H2O)m takes place at the high vacuum of the mass analyzer. The analyte molecule ions detected by MS
Atmospheric-pressure chemical ionization
Atmospheric-pressure_chemical_ionization
Diagnostic immunoassay using magnetic beads
moieties (fluorescent immunoassays) to detect a specified analyte. MIA involves the specific binding of an antibody to its antigen, where a magnetic label
Magnetic_immunoassay
determine the concentration of an identified analyte. The procedure involves preparing a particular reagent as a standard solution of known concentration
Glossary_of_chemistry_terms
Device detecting tiny amounts of explosives
observed from the excitation event. Since the exciton samples many potential analyte binding sites during its excited state lifetime, the probability that the
Explosives_trace_detector
CHCl3, historical anaesthetic and common solvent
Amylene has been found to be ineffective, and the phosgene can affect analytes in samples, lipids, and nucleic acids dissolved in or extracted with chloroform
Chloroform
For magnetic particle separations a droplet of solution containing the analyte of interest is placed on a digital microfluidics electrode array and moved
Digital_microfluidics
Routine laboratory test of blood cells
measuring cuvette and the absorbance is measured at a specific wavelength, which depends on the type of reagent used. A reference standard containing a known
Complete_blood_count
the 1960s. Ionization of sample (analyte) is achieved by interaction of its molecules with reagent ions. The analyte is ionized by ion-molecule reactions
History_of_mass_spectrometry
Scientific publications term
a chromatographic system there are three variables namely, the chiral analyte (CA), mobile phase and stationary phase, that can be manipulated to provide
Chiral_analysis
Analytical method using radiation to identify chemical elements in a sample
because each element has its own unique spectrum. The sample of a material (analyte) is brought into the flame as a gas, sprayed solution, or directly inserted
Atomic_emission_spectroscopy
Chromatographic method that uses a non-polar stationary phase
and analytes. Silica gel can be modified with various functional groups. Silica gel is cheap. Silica gel particles are easy to engineer into specific diameters
Reversed-phase_chromatography
Subdiscipline of chemistry concerned with chemical equilibrium
The analyte is dissolved in the mobile phase, and passes over the stationary phase. Separation occurs because of differing affinities of the analytes for
Equilibrium_chemistry
analyte. In forward phase arrays, each array is incubated with one test sample such as a cellular lysate or a patient's serum, but multiple analytes in
Reverse phase protein lysate microarray
Reverse_phase_protein_lysate_microarray
Synthesized biomolecular magnetic particles
preconcentrate the analyte and handled by a magnetic field or by modifying an electrode surface enhancing its conductivity and the affinity with the analyte. Coated-magnetic
Magnetic_nanoparticles
Material traceability inspection
Natural matrix CRMs (often shortened to 'matrix CRMs') contain an analyte or analytes in a natural sample (for, example, lead in fish tissue). These are
Certified_reference_materials
Analytical chemistry technique
protein in a given analyte mixture. Quantification is achieved through ion counts, the measurement of the amount of an ion at a specific retention time.
Quantitative_proteomics
Suspension of gold nanoparticles in a liquid
to as electron coupling. When the analyte and bio-receptor both bind to the gold, the apparent mass of the analyte increases and therefore amplifies the
Colloidal_gold
British biochemist
developed "stochastic sensing," which has been shown with a wide variety of analytes to reveal both concentration and identity through single-molecule detection
Hagan_Bayley
Mass of one equivalent
analyte (the species of interest). The different definitions came from the practice of quoting gravimetric results as mass fractions of the analyte,
Equivalent_weight
Purification technique for biomolecules
methods. Affinity chromatography has the advantage of specific binding interactions between the analyte of interest (normally dissolved in the mobile phase)
Affinity_chromatography
compounds, and quantitative analysis, by determining the concentration of the analytes of interest. Furthermore, it helps to determine different electrochemical
UV-Vis absorption spectroelectrochemistry
UV-Vis_absorption_spectroelectrochemistry
Aptamers, single-stranded RNA and DNA sequences, bind to an analyte and change their conformation. They function as nucleic acids selectively binding
Electrochemical aptamer-based biosensors
Electrochemical_aptamer-based_biosensors
Italian biotechnology company
multinational biotechnology company that produces and markets in vitro diagnostics reagent kits used in immunodiagnostics and molecular diagnostics and since July
DiaSorin
Interdisciplinary science
single chip, which enhances efficiency and mobility, and reduces sample and reagent volumes. The behaviour of fluids at the microscale can differ from "macrofluidic"
Microfluidics
Swiss medical technology company
and the consumable called the IVD CAPSULE, which is a single-use and analyte-specific tests. Test principle : The test is based on mixing the patient's sample
Abionic
Type of mass spectrometry that uses an inductively coupled plasma to ionize the sample
other forms of inorganic mass spectrometry is its ability to sample the analyte continuously, without interruption. This is in contrast to other forms
Inductively coupled plasma mass spectrometry
Inductively_coupled_plasma_mass_spectrometry
Ensuring accuracy of analytical measurements
validated. Identification tests are conducted to ensure the identity of an analyte in a sample through comparison of the sample to a reference standard through
Analytical_quality_control
Microtechnology
Microfluidic devices consume much smaller amounts of reagents, can be made to require only a small amount of analytes for chemical detection, require less time for
Bio-MEMS
Method for separation and analysis of biomolecules
visualized not only by general protein staining reagents but also by specific enzyme-linked staining. A specific experiment example of an application of native
Gel_electrophoresis
Technical analysis of a biological specimen
used to analyze the sample according to a recent publication wherein 12 analytes were investigated. Rapid oral fluid products are not approved for use in
Drug_test
Technique used in mass spectroscopy
contain on average one analyte ion or less. The gas-phase ions form after the remaining solvent molecules evaporate, leaving the analyte with the charges that
Electrospray_ionization
Red pigment of the bile
"Bilirubin". American Chemical Society. Retrieved 28 May 2021. Bilirubin: analyte monograph from The Association for Clinical Biochemistry and Laboratory
Bilirubin
Type of mass spectrometry
spectrometers use the first and third quadrupoles as mass filters. When analytes pass the second quadrupole, the fragmentation proceeds through collision
Tandem_mass_spectrometry
American scientist
multiple analytes, U.S. Patent Appl. Number: 20040231776 4. Stiso, S. N., Sutula, C. L., Method, composition and device for determining the specific gravity
Chester_L._Sutula
Chemical reaction
hydrogen atoms, so long as the substrate is robust to the conditions and reagents employed. This often results in perdeuteration: hydrogen-deuterium exchange
Hydrogen–deuterium_exchange
the titrant, and a = the corresponding number of moles reacting B = the analyte, and b = the corresponding number of moles reacting P = the product, and
Thermometric_titration
Concentration of glucose present in the blood (Glycaemia)
has media related to Blood sugar level. Glucose (blood, serum, plasma): analyte monograph – The Association for Clinical Biochemistry and Laboratory Medicine
Blood_sugar_level
Naturally produced monosaccharide
glucose itself. As a result of its importance in human health, glucose is an analyte in glucose tests that are common medical blood tests. Eating or fasting
Glucose
Catalyst participating in electrochemical reactions
coefficient, which compares the response of the material to the desired analyte or substrate with the response to other interferents. In many electrochemical
Electrocatalyst
Large-scale study of proteins
chromatin-associated proteins. Finally ICAT reagents are useful for proteomic profiling of cellular organelles and specific cellular fractions. Another quantitative
Proteomics
JR, Zhao L, Lin C, Yan P, Wang P, Paulovich AG. Sequential Multiplexed Analyte Quantification Using Peptide Immunoaffinity Enrichment Coupled to Mass
Stable isotope standards and capture by anti-peptide antibodies
Stable_isotope_standards_and_capture_by_anti-peptide_antibodies
Class of chemical substance
π–π interactions. This allows MOFs to isolate and concentrate selected analytes from complex samples before chromatographic or spectrometric detection
Metal–organic_framework
Lab technique in biology and chemistry
fluorescence intensity to distinguish multiple analytes in a single assay. The amount of the analyte captured is detected via a biotinylated antibody
Flow_cytometry
Concentration of organic carbon in a sample
inaccuracies associated with the addition of any foreign substance into the analyte and samples with high amounts of particulates. Performing "system blank"
Total_organic_carbon
Biochemical identification method
between a probed metal electrode and an electrolyte containing the target analyte. A known potential to the electrode is then applied from a feedback current
Fluorescent_tag
Analytical methodology in spectroscopy
Other requirements considered when designing operando experiments include reagent and product flow rates, catalyst position, beam paths, and window positions
Operando_spectroscopy
Variation in resonant frequency of identical atomic nuclei in a magnetic field
signal of the deuterated solvent is used and the solvents of reference and analyte are the same, the use of this methods is straightforward. Problems may
Chemical_shift
Device integrating laboratory functions on a integrated circuit
advantages, which are specific to their application. Typical advantages are: low fluid volumes consumption (less waste, lower reagents costs and less required
Lab-on-a-chip
μPAD-MS to enhance ionization efficiency (by enabling focusing of the analyte stream) and enable reaction mixing by wax printing on the triangular paper
Paper-based_microfluidics
environment Biosensor A biosensor is a device for the detection of an analyte that combines a biological component with a physicochemical detector component
Glossary_of_fuel_cell_terms
Non-invasive analytical tool
after the other—it is called heterogeneous assaying. The first analyte is linked to a specific binding agent on a solid support such as a polymer and then
Lanthanide_probes
method followed by IRMS detection. This is a 'compound-specific' method, allowing separation of analytes prior to measurement and thus providing information
Hydrogen isotope biogeochemistry
Hydrogen_isotope_biogeochemistry
Type of electrochemical cell
Integrating both anolyte and catholyte in the same molecule, i.e., bifunctional analytes or combi-molecules allow the same material to be used in both tanks. In
Flow_battery
Range of methods designed for assigning cell types
visualization of up to the whole transcriptome and 64 validated protein analytes and is the flexible, spatial single-cell imaging platform for cell atlasing
Spatial_transcriptomics
Methods for checking conformance to requirements
test passes and ensures the subsequent data collected for the unknown analytes are valid. For a longer HPLC run of over 20 samples, an additional system
Verification_and_validation
Chemical compound and essential nutrient
chemical modification to use is responsible for the biotin reagent binding to a specific protein. Second, the sample is incubated with avidin bound to
Biotin
Particle with size less than 100 nm
to isolate and remove undesirable proteins while enhancing the target analytes. Nucleation lays the foundation for the nanoparticle synthesis. Initial
Nanoparticle
create novel biosensors. These biosensors have been designed for detecting analytes such as hormones (e.g., progesterone) and addictive substances (e.g., nicotine)
Mark_W._Grinstaff
Alternative to the polymerase chain reaction
addition of further primer/probe pairs, allowing the detection of multiple analytes or an internal control in the same tube. RPA is one of several isothermal
Recombinase polymerase amplification
Recombinase_polymerase_amplification
Emission of light by a substance that has absorbed light
everywhere in the flow and differential quenching of their fluorescence by analytes is directly observed. Fluorescence-based assays for screening toxic chemicals
Fluorescence
a more hydrophobic manner, the elution times increased for each of the analytes for the given range. The trend generally applies over the entire temperature
Thermoresponsive polymers in chromatography
Thermoresponsive_polymers_in_chromatography
Type of chemical synthesis
parallel and evaluated through their binding constants to the imprinted analytes. Furthermore, adding another approach to the repertoire, Long et al. demonstrated
Automated_synthesis
ANALYTE SPECIFIC-REAGENT
ANALYTE SPECIFIC-REAGENT
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ANALYTE SPECIFIC-REAGENT
ANALYTE SPECIFIC-REAGENT
ANALYTE SPECIFIC-REAGENT
ANALYTE SPECIFIC-REAGENT
ANALYTE SPECIFIC-REAGENT
ANALYTE SPECIFIC-REAGENT