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3DISCO

• 3DISCO

3DISCO (stands for "3D imaging of solvent-cleared organs") is a histological method that make biological samples more transparent (so called "cleared")

3DISCO



3DISCO

• CUBIC

their high viscosity. Also, in comparison with solvent based methods like 3DISCO the CUBIC as method based on simple diffusion is still take slightly longer

CUBIC

CUBIC

• CLARITY

Method of making biological tissue transparent

found in gels used for western blots. Brainbow Diffusion Tensor Imaging 3DISCO Chung K, Wallace J, Kim SY, Kalyanasundaram S, Andalman AS, Davidson TJ

CLARITY

CLARITY

• Light sheet fluorescence microscopy

Fluorescence microscopy technique

Special tissue clearing techniques have been developed for this purpose, e.g. 3DISCO, CUBIC and CLARITY. Depending on the index of refraction of the cleared

Light sheet fluorescence microscopy



Light_sheet_fluorescence_microscopy

• Ali Ertürk

(November 2012). "Three-dimensional imaging of solvent-cleared organs using 3DISCO". Nature Protocols. 7 (11): 1983–1995. doi:10.1038/nprot.2012.119. ISSN 1750-2799

Ali Ertürk



Ali_Ertürk

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3DISCO

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3DISCO